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KMID : 1134820120410091220
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 9 p.1220 ~ p.1225
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Study on Antioxidant Effects of Fractional Extracts from Ligularia stenocephala Leaves
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Kim Kyoung-Hee
Kim Na-Young
Kim Sung-Hwan
Han In-Ae
Yook Hong-Sun
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Abstract
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Ligularia stenocephala leaves were subjected to extraction by using several solvents with different polarities, which were then investigated for their antioxidant and antimicrobial activities. Extraction yield of ethanol extract of Ligularia stenocephala was 21.36%. The water fraction showed the highest extraction yield of 60.35%, followed by the n-butanol, n-hexane, and ethyl acetate fractions. Total polyphenolic content was the highest (254.00 mg/g GAE) in the ethyl acetate fraction of Ligularia stenocephala extract. The ethyl acetate fraction had IC50 values of 0.28 mg/mL for DPPH radical scavenging, and 96.67±0.09% for ABTS radical scavenging activity. Further, FRAP value was significantly higher in the ethyl acetate fraction. The ethyl acetate fraction showed antimicrobial activities against B. cereus, and the diameter of the zone of inhibition was 10.2 mm at 5 mg/disc. These results suggest that the ethyl acetate fraction of Ligularia stenocephala possesses the antimicrobial activities against B. cereus, and its high antioxidant activity could be applicable to food additives and as a natural cosmetic ingredient.
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KEYWORD
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Ligularia stenocephala leaves, antioxidant, antimicrobial
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